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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has actually been observed in several tumors, which ends up in chromosome instability, and thus may possibly contribute to tumorigenesis. This protein was discovered to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a important function in endorsing cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been described. Two more splice variants were documented but in-depth nucleotide sequence info is just not still available.

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Blocking peptides are peptides that bind exclusively for the goal antibody and block antibody binding. These peptide commonly is made up of the epitope regarded by the antibody.

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This manual outlines more attainable optimization of cellular and immunoassay parameters to be sure the very best results are...

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This guide outlines further doable optimization of mobile and immunoassay parameters to guarantee the absolute best final results are...

Antibodies certain to the blocking peptide not bind on the epitope to the target protein. By evaluating the staining in the blocked antibody versus the antibody by itself, you can see which staining is certain.

Concentrate on Info Cyclin E belongs for the really conserved cyclin family, whose users are characterised by a spectacular periodicity in protein abundance through the cell cycle. Cyclins purpose as regulators of CDK kinases. Different cyclins exhibit distinctive expression and degradation designs which contribute towards the temporal coordination of each and every mitotic celebration. Cyclin E sorts a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S transition. This protein accumulates on the G1-S phase boundary and it is degraded as cells progress by way of S stage.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...

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